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This site gives you general information on how and why molecular techniques work. Here are links to some sites that give you specific protocols for performing individual techniques.
- BIOSCI. A set of electronic communication forums—the bionet USENET newsgroups and parallel e-mail lists—used by biological scientists worldwide. No fees are charged for the service.
http://www.bio.net/
- Cell and Molecular Biology Online’s methods and protocols page.
http://www.cellbio.com/protocols.html - Comprehensive protocol collection. From the Ambrose lab at Dartmouth College.
http://cobweb.dartmouth.edu/~ambros/molbio/index.html - http://www.uct.ac.za/depts/mmi/bbhelp/con2.html#CON_PRX_MAN
- Hugh Griffin’s site. This site will link you to a variety of molecular biology pages, including many protocols. This page has a large number of sponsor banners, so be prepared for it to load a little slower.
http://www.highveld.com/protocols.html
- Mark Strom’s Molecular Biology Protocols site. Mark Strom at the Microbial Pathogenesis/Utilization Research Division, Northwest Fisheries Science Center (NMFS/NOAA). Lots of protocols and helpful hints.
http://research.nwfsc.noaa.gov/protocols.html
- Molecular Biology Techniques Manual. An excerpt from the hardcopy version. Includes descriptions and protocols for SDS-PAGE, PCR, and Western blotting. Lots of highly technical information about these techniques.
http://www.cellbio.com/protocols.html - PCR and multiplex PCR: guide and troubleshooting. An online PRC manual put together by Octavian Henegariu of Yale University.
http://info.med.yale.edu/genetics/ward/tavi/PCR.html - Protocol Online. A database that organizes many online protocol resources. This site also has discussion forums.
http://www.protocol-online.net/ - Protocols for recombinant DNA isolation, cloning, and sequencing. An extensive online manual of everyday lab techniques. Put together by Bruce A. Roe, Judy S. Crabtree, and Akbar S. Khan, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman.
http://dna1.chem.ou.edu/protocol_book/protocol_index.html - Protocols from the Crawford Laboratory at the University of Missouri, Kansas City.
http://sgi.bls.umkc.edu/funnylab/DLCprotocols.html - Technical Tips Online. A peer-reviewed site that includes protocols, handy tips, and product information.
http://tto.trends.com/tto/index_of_TTO.shtml
